Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (1): 68-73.doi: 10.3969/j.issn.1673-8225.2011. 01.015

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Influence of stromal cell-derived factor-1 alpha and its receptor CXCR4 on vascular smooth muscle cells and bone marrow stromal cells in common carotid artery balloon injury models  

Cai Wen-wei1, Fang Ning-yuan2, Sheng Jing1, Ma Shao-jun1, Cheng Zhi-hui1   

  1. 1Department of Geriatrics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200011, China
    2Department of Geriatrics, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200127, China
  • Received:2010-07-23 Revised:2010-09-20 Online:2011-01-01 Published:2011-01-01
  • Contact: Sheng Jing, Master, Chief physician, Department of Geriatrics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China shj-60@hotmail.com
  • About author:Cai Wen-wei☆, Doctor, Associate chief physician, Department of Geriatrics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China alonhuc@sohu.com
  • Supported by:

    the Grant of Health Bureau of Shanghai City, No. 2008088

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Abstract:

BACKGROUND: Bone marrow stromal cells (BMSCs) can differentiate into endothelial progenitor cells by the action of cytokine and migrate to the injured site to participate in vascular intima repair.
OBJECTIVE: To observe the influence of the interaction between stromal cell-derived factor-1α (SDF-1α) and its receptor CXCR4 on vascular smooth muscle cells and BMSCs in the rat common carotid artery balloon injury model.
METHODS: Vascular smooth muscle cells and BMSCs were cultured in vitro according to different time points just after surgery (0), 1, 4, 7, 14 days and 1 month after balloon injury in rat common carotid artery. SDF-1α mRNA expressions were detected in vascular smooth muscle cells while CXCR4 mRNA expressions were detected in BMSCs. SDF-1α siRNA was transfected into vascular smooth muscle cells and transwell body was used to measure the chemotaxis of SDF-1α or vascular smooth muscle cells to BMSCs.
RESULTS AND CONCLUSION: SDF-1α mRNA expression began to increase just after balloon injury, and then subsided gradually on day 7. CXCR4 mRNA began to express on day 4 after balloon injury and lasted. Transwell cabin demonstrated that SDF-1 chemotaxis increased in injured BMSCs than in normal ones; the antagonist of CXCR4 receptor AMD3100 could weaken SDF-1 chemotaxis significantly. The chemotaxis of injured vascular smooth muscle cells to BMSCs was stronger than SDF-1 (P < 0.05) and the action could be weakened by AMD3100 or transfection of SDF-1α siRNA in vascular smooth muscle cells (P < 0.05). Results indicated that SDF-1α/CXCR4 should be important in the process of BMSCs migration to injured vessels.

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